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Features

• Ultrapure quality
• Non-specific blood group activity
• Sugar specificity: Gal – GalNAc

Product description

Wisteria floribunda lectin is isolated from japanese wisteria seeds and purified by affinity chromatography. The lectin has a molecular weight of 116 kDa and is composed of four polypeptide chains of 29 kDa.

The lectin labels selectively N-acetylgalactosamines beta 1 (GalNAc beta 1-3 Gal) residues of glycoproteins within the extracellular matrix of the neurons, and has been identified as a specific marker for functionally different cortical areas of the rodent brain (1).

Medicago’s Wisteria floribunda lectin is supplied as a white to yellowish-white lyophilized powder. The purity is determined by SDS- PAGE, which generates one single band at 29 kDa corresponding to the four polypeptide chains. The lectin is available in vials containing 5 mg or 25 mg or 100 mg powder.

The product is to be used for laboratory work only.

Applications

• Studies of virus surface glycoproteins
• Purifying virus when immobilized an agarose gel

Directions for use

The lectin may be reconstituted with the buffer of choise, spin the vial gently until full dissolution.

Tips and hints

Avoid repeated freezing and thawing.

Shipping and storage

The product is shipped at -20°C however for over-theday transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months

References

(1) Anat Embryol (Berl). 2001 Jan;203(1):45-52. Wisteria floribunda agglutinin labeling patterns in the human cortex: a tool for revealing areal borders and subdivisions in parallel with immunocytochemistry. Hilbig H1, Bidmon HJ, Blohm U, Zilles K. (2) Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[ beta 14]GlcNAc), by Wisteria floribunda Agglutinin. Haji-Ghassemi, O., Gilbert, M., Spence, J., Schur, M.J., Parker, M.J., Jenkins, M.L., Burke, J.E., van Faassen, H., Young, N.M., Evans, S.V.