*** For pack size 1g or more, please contact us to inquire about pricing





Features

• Ultrapure quality
• Sugar specificity: N-acetyl galactoseamine, galactose, complex oligosaccharides containing N-acetylgalactoseamine
• Haemagglutinating activity
• Lyophilized powder

Product description

"Wisteria floribunda lectin is isolated from japanese wisteria seeds and purified by affinity chromatography. The lectin has a molecular weight of 116 kDa and is composed of four polypeptide chains of 29 kDa.

The lectin labels selectively N-acetylgalactosamines beta 1 (GalNAc beta 1-3 Gal) residues of glycoproteins within the extracellular matrix of the neurons, and has been identified as a specific marker for functionally different cortical areas of the rodent brain. Wisteria floribunda lectin can also be used as a probe for serum biomarkers of several diseases such as liver fibrosis, liver cirrosis, IgA nephropathy, entometriosis and a long list of cancer types, with very low background noise.

Medicago’s Wisteria floribunda lectin is supplied as a white to yellowish-white lyophilized powder. The purity is determined by SDS- PAGE, which generates one single band at 29 kDa corresponding to the four polypeptide chains. The lectin is available in vials containing 5 mg or 100 mg powder, but for bulk purchases other packaging can be prepared. The product is to be used for laboratory work only."

Applications

• Studies of glycoproteins
• Histochemistry in neural tissus
• Detection and isolation of serum biomarkers for several diseases
•  Affinity chromatography
  
  
  

Directions for use

The lectin may be reconstituted with PBS or equivalent buffers, spin the vial gently until full dissolution.

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with PBS, the solution may be stored frozen in working aliquots for up to 12 months.

References

Forostyak S, Homola A, Turnovcova K, Svitil P, Jendelová P, Syková E. (2014). Intrathecal Delivery of Mesenchymal Stromal Cells Protects the Structure of Altered Perineuronal Nets in SOD1 Rats and Amends the Course of ALS. STEM CELLS. 32. 10.1002/stem.1812.

Hilbig H, Bidmon HJ, Blohm U, Zilles K. Wisteria floribunda agglutinin labeling patterns in the human cortex: a tool for revealing areal borders and subdivisions in parallel with immunocytochemistry. Anat Embryol (Berl). 2001 Jan;203(1):45-52. doi: 10.1007/s004290000135

Narimatsu H, Sato T. (2018) Wisteria floribunda agglutinin positive glycobiomarkers: a unique lectin as a serum biomarker probe in various diseases, Expert Review of Proteomics, 15:2, 183-190, DOI: 10.1080/14789450.2018.1419066

MSDS: 

MSDS - swedish

MSDS - english

MSDS - deutsch