Features

• Running buffers for protein electrophoresis
• Formulated from analytical grade reagents
• Assured consistency from lot to lot
• Exactly pre-weighed powder mix in pouches
• Dissolve and use in minutes                 

Product description

Tris-glycine-SDS buffer (TG-SDS) contains the denaturing agent sodium dodecyl sulphate (SDS). Protein electrophoresis under denaturing conditions (SDS-PAGE) involves separating proteins based on their size. By treating the sample under denaturing and reducing conditions with SDS, proteins unfold and become coated with SDS detergent molecules.

Medicago’s TG-SDS buffer is supplied as pre-weighed powder mixes in sealed pouches giving 1000 ml or 5000 ml of 0.025 M Tris, 0.192 M glycine, 0.10% SDS with pH 8.3 at 25°C.

Applications

• Protein electrophoresis
• Denatured protein electrophoresis
• Polyacrylamide gel electrophoresis
• Western blotting

Directions for use

Empty one pouch of TG-SDS buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes.
Adjust the volume up to 1000 ml or 5000 ml, stir until full dissolution and the buffer solution is ready to use.

Shipping and storage

The TG-SDS buffers are shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

Specifications                                  

Chemicals: Analytical grade

Format: Exactly pre-weighed powder mix

Concentration: 0.025 M Tris, 0.192 M glycine, 0.10% SDS

Volume: 1000 ml and 5000 ml                 

pH: 8.3 ± 0.2 at 25°C

MSDS: 

MSDS - swedish