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Tris-Borate-EDTA (TBE) Buffer pH 8.3
Features
- Nucleic acid electrophoresis buffers
- Molecular biology grade
- TBE stock solutions 1x, 5x and 10x
- Exactly pre-weighed in pouches
- Dissolve and use in minutes
Product description
In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel).
TAE is advantageous for high resolution of long nucleic acid
fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA
analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide.
Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000 ml of 1x, 5x or 10x Tris-borate-EDTA buffer or 50x Tris-acetate-EDTA buffer with pH 8.3 at 25°C.
Applications
- Nucleic acid electrophoresis running buffer for for agarose and polyacrylamide gels
- Native and denaturing RNA analysis
- Northern blotting
Directions for use
Empty one pouch of the TAE or TBE buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the buffer solution is ready to use.
Shipping and storage
TBE and TAE buffers are shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.
Specifications
| TBE 1x | TBE 5x | TBE 10x | ||
| Chemicals | Analytical grade | Analytical grade | Analytical grade | |
| RNAse/DNase activity | Non-detectable | Non-detectable | Non-detectable | |
| Format |
Exactly pre-weighed |
Exactly pre-weighed |
Exactly pre-weighed |
|
| Composition |
0.089 M Tris-Borate
|
0.445 M Tris-Borate 0.010 M EDTA |
0.89 M Tris-Borate 0.020 M EDTA |
|
| Volume | 1000 ml | 1000 ml | 500 ml | |
| pH | 8.3 ± 0.15 at 25°C (1x solution) | 8.3 ± 0.15 at 25°C (1x solution) | 8.3 ± 0.15 at 25°C (1x solution) | |
