Tris-Glycine (TG) Buffer pH 8.3
- Running buffers for protein electrophoresis
- Formulated from analytical grade reagents
- Assured consistency from lot to lot
- Exactly pre-weighed powder mix in pouches
- Dissolve and use in minutes
Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.
TG buffer is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.
Medicago’s TG buffer are supplied as pre-weighed powder mixes in sealed pouches giving 1000 ml or 5000 ml of 0.025 M Tris, 0.192 M glycine with pH 8.3 at 25°C.
- Protein electrophoresis
- Denatured protein electrophoresis
- Polyacrylamide gel electrophoresis
- Western blotting
Directions for use
Empty one pouch of the TG or TG-SDS buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes.
Adjust the volume up to 1000 ml or 5000 ml, stir until full dissolution and the buffer solution is ready to use.
Shipping and storage
The TG buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.
Chemicals: Analytical grade
Format: Exactly pre-weighed powder mix
Concentration: 0.025 M Tris, 0.192 M glycine
Volume: 1000 ml and 5000 ml
pH: 8.3 ± 0.2 at 25°C