SmartBuffers

Tris-Glycine-SDS (TG-SDS) Buffer pH 8.3

Article NoProduct namePack SizeSolution VolPriceQuantityBuy
Article No: 
12-9223-10
TG-SDS pH 8.3
Pack Size: 
10 pouches
Solution Vol: 
5000 ml/pouch
Price: 107 EUR
Article No: 
12-9222-10
TG-SDS pH 8.3
Pack Size: 
10 pouches
Solution Vol: 
1000 ml/pouch
Price: 49 EUR
TG-SDS

Features

  • Running buffers for protein electrophoresis
  • Formulated from analytical grade reagents
  • Assured consistency from lot to lot
  • Exactly pre-weighed powder mix in pouches
  • Dissolve and use in minutes                 

 

 

 

Product description

Tris-glycine-SDS buffer (TG-SDS) contains the denaturing agent sodium dodecyl sulphate (SDS). Protein electrophoresis under denaturing conditions (SDS-PAGE) involves separating proteins based on their size. By treating the sample under denaturing and reducing conditions with SDS, proteins unfold and become coated with SDS detergent molecules.

Medicago’s TG-SDS buffer is supplied as pre-weighed powder mixes in sealed pouches giving 1000 ml or 5000 ml of 0.025 M Tris, 0.192 M glycine, 0.10% SDS with pH 8.3 at 25°C.

 

Applications

  • Protein electrophoresis
  • Denatured protein electrophoresis
  • Polyacrylamide gel electrophoresis
  • Western blotting

 

Directions for use

Empty one pouch of TG-SDS buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes.
Adjust the volume up to 1000 ml or 5000 ml, stir until full dissolution and the buffer solution is ready to use.

 

Shipping and storage

The TG-SDS buffers are shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications                                  

Chemicals: Analytical grade

Format: Exactly pre-weighed powder mix

Concentration: 0.025 M Tris, 0.192 M glycine, 0.10% SDS

Volume: 1000 ml and 5000 ml                 

pH: 8.3 ± 0.2 at 25°C

MSDS: