SmartBuffers

Tris-Acetate-EDTA (TAE) Buffer 50x, pH 8.3

Article NoProduct namePack SizeSolution VolPriceQuantityBuy
Article No: 
12-9144-5
TAE 50x
Pack Size: 
5 pouches
Solution Vol: 
1000 ml/pouch
Price: 429 EUR
Article No: 
12-9145-5
TAE 50x
Pack Size: 
5 pouches
Solution Vol: 
500 ml/pouch
Price: 240 EUR
TAE buffer

Features

  • Nucleic acid electrophoresis buffers
  • Molecular biology grade
  • TBE stock solutions 1x, 5x and 10x, TAE stock solution 50x
  • Exactly pre-weighed in pouches
  • Dissolve and use in minutes           

 

 

 

Product description

In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel).

TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster).  TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide.

Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000 ml of 1x, 5x or 10x Tris-borate-EDTA buffer or 50x Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

 

Applications

  • Nucleic acid electrophoresis running buffer for for agarose and polyacrylamide gels
  • Native and denaturing RNA analysis
  • Northern blotting

 

Directions for use

Empty one pouch of the TAE or TBE buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the buffer solution is ready to use.

 

Shipping and storage

TBE and TAE buffers are shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

RNAse/DNase activity: Non-detectable

Format: Exactly pre-weighed powder mix

Composition: 2.0 M Tris-acetate, 0.050 M EDTA

Volume: 500 ml and 1000 ml

pH: 8.3 ± 0.1 at 25°C (50x solution)