SmartBuffers

Phosphate Buffered Saline (PBS) pH 7.4 and 7.2

Article NoProduct namePack SizeSolution VolPriceQuantityBuy
Article No: 
12-9425-1
PBS pouches pH 7.4
Pack Size: 
1 pouch
Solution Vol: 
50 l/pouch
Price: 70 EUR
Article No: 
12-9422-1
PBS pouches pH 7.4
Pack Size: 
1 pouch
Solution Vol: 
25 l/pouch
Price: 45 EUR
Article No: 
12-9426-1
PBS pouches pH 7.4
Pack Size: 
1 pouch
Solution Vol: 
100 l/pouch
Price: 115 EUR
Article No: 
10-9402-10
PBS solution pH 7.4
Pack Size: 
10 bottles
Solution Vol: 
1000 ml/bottle
Price: 111 EUR
Article No: 
09-9499-100
PBS tablets pH 7.2
Pack Size: 
100 tablets
Solution Vol: 
1000 ml/tablet
Price: 208 EUR
Article No: 
09-9499-10
PBS tablets pH 7.2
Pack Size: 
10 tablets
Solution Vol: 
1000 ml/tablet
Price: 42 EUR
Article No: 
12-9424-1
PBS pouches pH 7.4
Pack Size: 
1 pouch
Solution Vol: 
10 l/pouch
Price: 21 EUR
Article No: 
12-9423-5
PBS 10x pouches pH 7.4
Pack Size: 
5 pouches
Solution Vol: 
1000 ml/pouch
Price: 55 EUR
Article No: 
09-2052-100
PBS tablets pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
200 ml/tablet
Price: 54 EUR
Article No: 
09-2051-100
PBS tablets pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
100 ml/tablet
Price: 32 EUR
Article No: 
09-8912-100
PBS tablets pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
500 ml/tablet
Price: 116 EUR
Article No: 
09-8912-12
PBS tablets pH 7.4
Pack Size: 
12 tablets
Solution Vol: 
500 ml/tablet
Price: 27 EUR
Article No: 
09-9400-100
PBS tablets pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
1000 ml/tablet
Price: 208 EUR
Article No: 
09-9400-10
PBS tablets pH 7.4
Pack Size: 
10 tablets
Solution Vol: 
1000 ml/tablet
Price: 42 EUR
PBS Tablet

Features

  • Formulated from analytical grade reagents
  • Autoclavable
  • Choice of two compositions: with or without potassium
  • Available as convenient tablets or powder mix in pouches
  • Ready to use in minutes

 

 

 

 

 

Product description

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns (1).

Medicago’s PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches.
There are seventeen standard-sized packages and final volumes range from 100 ml to 100 litres. Choose from different pH, 7.2 and 7.4 and from PBS with or without potassium. PBS without potassium is available in two different molar strengths, 0.01 M and 0.02 M. PBS solution is supplied as a ready-to-use solution.

 

Applications

  • Immunoassays
  • Immuno-histochemical procedures
  • Microbiological procedures
  • Tissue and cell culture procedures
  • Sample dilution

 

Directions for use

Empty one pouch or deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml to 500 ml of deionized water for pouches and tablets in the volume range 100 ml to 1000 ml and stir the solution for a few minutes. Adjust the water up to the given volume, stir until full dissolution and the buffer is ready to use. For larger quantities, 10 to 100 litres of buffer, use suitable equipment to stir and dissolve the buffer in compliance with the instructions above.

 

Shipping and storage

PBS buffer is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.. Medicago’s ready-to-use PBS solution in bottles is stable for one year at +4°C.

 

References

1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz.Nature Biotechnology 19, 1173 - 1176.