Phaseolus vulgaris lectin M (PHA-M)
• Two grades of purity; PHA-M pure and PHA-M crude
• Potent mitogen
• High erythroagglutinating activity
• Stimulation of cell proliferation in lymphocyte cultures • Erythroagglutination
PHA-M is the mucoprotein variant of phytohemaglutinin isolated from red kidney bean. It contains up to 20% carbohydrates in conjugation with the protein which presents two bands corresponding to about 35 kDa according to SDS-PAGE in presence of mercaptoethanol. It is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures and also has a powerful erythroagglutinating activity.
Storage and stability
Stable for at least 3 years from production date when stored below -20 ̊C. May be shipped at ambient temperature. After reconstitution in PBS pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months. Repeated freez- ing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.