EC-Blue Enhanced™ Substrate (TMB)
- Ready-to-use liquid substrate
- Substrate for assays using horseradish peroxidase (HRP) conjugate
- Low background
- High sensitivity
- Long stability
3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).
One bottle of EC Blue consists of a solution containing proprietary ready-mixed chromogen substrate reagents, pH 3.8. The solution has a straw-yellow colour at room temperature and a light greenish tint when stored at 4°C. The colour returns to normal when warmed overnight to room temperature.
For laboratory use only.
- Recommended for ELISA procedures
Storage and stability
Store at 2°C to 8°C. Do not freeze and do not expose to direct sunlight. Prolonged exposure to light is not recommended. Store in an amber bottle. As some metal ions can oxidize TMB, allow only glass or plastic to come in contact with the substrate.
Never pipette directly from the bottle. Do not leave the cap off. EC-Blue Enhanced™ Reagent is stable for at least 18 months when stored at 2°C to 8°C.
Directions for use
No additional mixing reagents are required. The material is not dependent on room temperature.
Shipping and Storage
The product is stable for 12 months if stored at 2°c to 8°C.
Appearance: Straw yellow colour at room temperature. Slightly green tint when stored at +4°C. Colour returns to yellow when stored at room temperature.
Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.
Functional characteristics: Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.
Storage: 2°C to 8°C