SmartReagents

Sodium Dodecyl Sulphate Reagent (SDS)

Article NoProduct namePack SizeSolution VolPriceQuantityBuy
Article No: 
09-2026-50
SDS
Pack Size: 
50 tablets
Solution Vol: 
0.5 g/tablet
Price: 26 EUR
Article No: 
12-9193-5
SDS 10%
Pack Size: 
5 pouches
Solution Vol: 
1000 ml/pouch
Price: 357 EUR
Article No: 
12-9194-5
SDS 20%
Pack Size: 
5 pouches
Solution Vol: 
1000 ml/pouch
Price: 614 EUR
SDS Reagent

Features            

  • Prepared from analytical grade reagent           
  • Anionic detergent
  • Denaturing agent used in SDS-PAGE
  • Choice of tablets and powder in pouches
  • Dissolve and use in minutes

 

 

 

Product description

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain. As a result, the distance of migration through the gel in SDS-PAGE electrophoresis can be assumed to be directly related only to the size of the protein.

Medicago’s SDS reagent is supplied in pre-weighed tablets. One tablet weighs 0.5 g and can be dissolved in deionized water to the desired volume and concentration. SDS reagent powder mix in pouches give 1000 ml of 10% or 20% sodium dodecyl sulphate solution.

 

Applications

  • Solubilizes and denatures proteins
  • SDS-PAGE electrophoresis
  • Western blots

 

Directions for use

Empty one pouch or deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the solution is ready to use.

Deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes until full dissolution. Adjust the volume up to required concentratio and the solution is ready to use.

 

Shipping and storage

The SDS reagent is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets or powder

Concentration: 10% or 20% solution

Weight: 0.5 g/tablet

Volume: 1000 ml