Saline Sodium Citrate (SSC) Buffer pH 7.0
- Formulated from analytical grade chemicals
- Two stock solutions: 2x and 20x
- Buffering agent to control acidity
- Eliminates tedious pH adjustments
Medicago’s SSC buffer is specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. SSC buffer is an acid salt buffer solution and is used as a buffering agent to control acidity. The buffer is supplied in two sizes giving 100 ml or 1000 ml of 0.30 M sodium chloride, 0.030 M trisodium citrate buffer or 1000 ml of 3.0 M sodium chloride, 0.30 M trisodium citrate buffer with pH 7.0 at 25°C when the contents of one pouch is dissolved in deionized water.
- Nucleic acid hybridization
- Blot transfer procedures
- Nucleic acid preparation
Directions for use
Empty one pouch of the SSC buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml or 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 100 ml or 1000 ml, stir until full dissolution and the buffer stock solution is ready to use.
Shipping and storage
SSC buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date. Stock solutions can be stored at room temperature or at +2°C to +8°C for two months.
Chemicals: Analytical grade
Format: Exactly pre-weighed powder mix and tablets
Composition 20x: 3.0 M NaCl buffer, 0.30 M trisodium citrate
Composition 2x: 0.30 M NaCl buffer, 0.030 M trisodium citrate
Volume: 100 ml and 1000 ml
pH: 7.0 ± 0.05 at 25°C