- Pure recombinant Protein A
- Immobilized on a cross-linked agarose matrix
- Strong binding to Fc region of IgG
- High capacity: > 20 mg IgG/ml
Immunosorb A consists of highly purified recombinant Protein A which has been derived from the bacterium Staphylococcus aureus. The protein is cloned and expressed in E.coli and immobilized on a cross-linked agarose matrix.
Protein A binds to specific classes of immunoglobulins and can preferably be used for purification of mouse and rabbit immunoglobulins. The protein binds to the Fc region of immunoglobulins by interacting with the heavy chain. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1 but with moderate affinity. It does not react with human IgG3 or IgD, nor with mouse IgM, IgA or IgE.
The material is non-cultural, non-infectious and is not pathogenic for animals or plants. It does not contain any animal or cell culture derived products or additions such as albumin or serum nor any toxic or harmful substances.
Medicago’s Immunosorb A is provided as a liquid solution with 20 % ethanol as preservative in pack sizes 100 ml, 200 ml and 5 ml.
For research use only.
- Purification of human, mouse and rabbit immunoglobulins
- Immobilization and detection of immunoglobulins
- Affinity chromatography
Directions for use
No additional mixing reagents are required. The material is not dependent on room temperature.
Shipping and storage
The product is shipped at room temperature and is stable for 12 months if stored at 2°c to 8°C.
Appearance: White liquid solution
Source: Cloned and expressed in E.coli
Purity: > 95%
Preservatives: 20% ethanol
Binding specificity: Protein A binds to specific classes of immunoglobulins